As I mentioned in the litter announcement video, this is an intentionally dual-sired litter and we will use DNA to establish paternity.
Sapphire
The Gems’ DNA samples arrived at the lab last week, and I hope we will have results by mid-July 🍀
Jasper
AKC DNA Analysis generates results for 201 markers in the form of letters. For each marker, a dog has two letters — one from their mom and one from their dad. Here is what it looks like in the report.
AKC will do the analysis of paternity for $50 and I will have that done to validate results, but this is not actually rocket science.
Star Garnet
I made a spreadsheet to do the paternity analysis. I eliminated all DNA markers in which the potential sires are the same. If they are both AA, for example, and Capella is TT, it tells us nothing if the puppy is AT since the A could have come from either sire.
Topaz and Sapphire
The pink highlighted are the markers that went into the spreadsheet because the two potential dads have different results for those markers.
And here is a snippet from the spreadsheet.
For DNA Marker #1, Capella will always contribute one of her C’s. The same is true for Ash — he can only contribute C. But Deuce can only contribute an A and this means Marker #1 will tell the story, right?
Jasper
If the puppy is CC, Ash has to be the dad. If the puppy is AC, Deuce must be the dad.
Sapphire with Star Garnet behind
Marker #2 is not as definitive. If the puppy is AA, that will mean Capella contributed her A but we cannot know which potential sire contributed the other one. If the puppy is AG, we do not know if the G came from Capella or Ash. But if the puppy is GG then we can rule out Deuce as the sire because he doesn’t have a G to contribute.
I think it is all so very interesting!
And he’s out…
🤷🏼♀️
Happy Tuesday 🩷💙💙💙